GSM1595666: Testis; Takifugu rubripes; miRNA-Seq - SRA

SRX857212: GSM1595666: Testis; Takifugu rubripes; miRNA-Seq
2 ABI_SOLID (AB SOLiD System 3.0) runs: 24.6M spots, 861.9M bases, 723.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Deep sequencing, profiling and detailed annotation of miRNAs in Takifugu rubripes

PRJNA273883 • SRP052920 • All experiments • All runs

show Abstracthide Abstract

We performed extensive transcriptome analysis of the tiger pufferfish (Takifugu rubripes) using the next-generation sequencer SOLiD III, and profiled millions of small RNA sequences in somatic tissues and gonads, as well as identifying thousands of miRNAs. The number of miRNAs identified was the highest in fish species reported to date. We also report highly variable isomiR features. Overall design: miRNA expression profiles of 9 tissues (7-somatic tissues and 2-gonads) of torafugu were generated by deep sequencing using SOLiD3.

Sample: Testis

SAMN03299892 • SRS829204 • All experiments • All runs

Organism: Takifugu rubripes

Library:

Instrument: AB SOLiD System 3.0

Strategy: miRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: Total RNA was extracted using a mirVana miRNA Isolation Kit (Applied Biosystems). Nine small RNA libraries were constructed, using Small 415 RNA Library Preparation for SOLiD Sequencing (Applied Biosystems)

Experiment attributes:

GEO Accession: GSM1595666

Links:

External link: GEO Sample GSM1595666

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 24.6M spots, 861.9M bases, 723.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR1777562	12,203,245	427.1M	357.6Mb	2016-01-28
SRR1777563	12,421,114	434.7M	365.8Mb	2016-01-28

ID:: 1207939

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Sequence Read Archive

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