show Abstracthide AbstractWe collected ten embryonic stages covering cleavage, blastula, gastrula, somite, and late stages until hatching. We also collected two adult tissue samples, including ovary and testis. 13 samples were sequenced using the illumina short-read sequencing platform. Overall design: Total RNA was extracted from medaka whole embryo samples of 11 developmental stages and 2 adult tissues. Short-read strand-specific library was constructed and sequenced using Illumina platform.