GSM4521199: angii1; Mus musculus; RNA-Seq - SRA

SRX8287721: GSM4521199: angii1; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 30.5M spots, 4.6G bases, 1.8Gb downloads

Submitted by: NCBI (GEO)

Study: RNA sequencing study using Prox1-eGFP mice to investigate the role of lymphatic vasculature in chronic cardiac dysfunction

PRJNA630980 • SRP260447 • All experiments • All runs

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The goal of the study is to examine the transcriptional changes in different cell populations isolated from heart and diaphragm in angiotensin II infusion-induced cardiac dysfunction and with a lymphangiogenic therapy (VEGFCc156s) Overall design: Prox1-eGFP mice were treated with BSA, angiotensin II in BSA or angiotensin II and VEGFCc156s for 5-6 weeks and used for the isolation of different cell populations from heart and diaphragm. The transcriptional profile of isolated cell populations was examined using RNA-Seq.

Sample: angii1

SAMN14848513 • SRS6609538 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cardiomyocytes, lymphatic endothelial cells and blood endothelial cells were isolated from mouse heart and lymphatic endothelial cells were isolated from mouse diaphragm using an optimized protocol with FACS Total RNA was converted into cDNA using the Smart-Seq ultra low input RNA kit and cDNA was converted into libraries using Nextera DNA XT Library Prep Kit

Experiment attributes:

GEO Accession: GSM4521199

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 30.5M spots, 4.6G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR11728487	30,529,078	4.6G	1.8Gb	2020-11-16

ID:: 10790300

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