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SRX825461: GSM1574422: ZH2 rep2; Prunus persica; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 10.2M spots, 996.5M bases, 684.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Identification of Volatile and Softening-Related Genes Using Digital Gene Expression Profiles in Peach
show Abstracthide Abstract
Digital gene expression (DGE) profiles based on Illumina RNA-seq technology were applied to investigate the different gene expression patterns in peach fruit mesocarp of four commercial cultivars (‘Hu Jing Mi Lu’-‘HJ’, ‘Yu Lu’-‘YL’, ‘Zhong Hua Shou Tao’-‘ZH’ and ‘Jin Xiu Huang Tao’-‘JX’) and at three ripening stages of ‘HJ’. The aim was to identify the key candidate genes related to the formation of volatile compounds and fruit softening, and to get an insight into molecular mechanisms associated with these two traits from a full transcriptome view. The large set of differentially expressed genes (DEGs), and the annotation of each gene provides valuable information for research on other fruit traits Overall design: Twelve digital gene expression profiles based on Illumina RNA-seq technology were applied to investigate the gene expression pattern in peach fruit mesocarp of four commercial cultivars (‘Hu Jing Mi Lu’-‘HJ’, ‘Yu Lu’-‘YL’, ‘Zhong Hua Shou Tao’-‘ZH’ and ‘Jin Xiu Huang Tao’-‘JX’) and at three ripening stages of ‘HJ’. The firmness and the content of seven volatile compounds especially lactone and linalool was assessed to detect candidate genes which are associated with these two traits
Sample: ZH2 rep2
SAMN03273863 • SRS804999 • All experiments • All runs
Organism: Prunus persica
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA for each sample was isolated from 1g frozen fruit tissue, according to Zhang (2010). Contaminating genome DNA was removed by RNase-free DNase I (Fermentas, Vilnius, Lithuania). RNA integration and quantification was by agarose electrophoresis at an absorbance of 260nm (A260) using the NanoDrop® ND-3300 Fluorospectrometer with Quant-iT™ RiboGreen® RNA Reagent (Invitrogen, USA). First-strand cDNA was synthesized using 2.0 μl DNA-free RNA with the PrimeScript RT reagent Kit with gDNA Eraser, following the manufacturer’s instructions (Takara), and was diluted for RT-qPCR using a CFX 96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1574422
Links:
External link:
Runs: 1 run, 10.2M spots, 996.5M bases, 684.5Mb
Run# of Spots# of BasesSizePublished
SRR173652710,167,958996.5M684.5Mb2016-12-02

ID:
1175166

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