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SRX8181925: GSM4501360: siB/siM-3; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 37.5M spots, 7.6G bases, 2.7Gb downloads

Submitted by: NCBI (GEO)
Study: The transcriptional repressor, BCL11A, promotes breast cancer metastasis
PRJNA628687 • SRP258724 • All experiments • All runs
show Abstracthide Abstract
Identification of the BCL11A-regulated transcriptome and spliceosome and determination of MBNL1-dependent splicing changes by RNA-Seq Overall design: Two/Three replicates were analyzed for each experimental condition.
Sample: siB/siM-3
SAMN14738736 • SRS6540992 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated using the Rneasy Plus Mini Kit (Qiagen) and processed as per manufacturer's instructions. Illumina TruSeq Stranded Total RNA kit with Ribo Zero Gold for rRNA removal was used. Illumina TruSeq libraries were tagged with unique adapter-indexes. Final libraries were validated on the Agilent 2100 Bioanalyzer, quantified via qPCR (KAPA Biosystems Illumina Library Quantification Kit) and pooled at equimolar ratios
Experiment attributes:
GEO Accession: GSM4501360
Links:
Runs: 1 run, 37.5M spots, 7.6G bases, 2.7Gb
Run# of Spots# of BasesSizePublished
SRR1161574337,455,4557.6G2.7Gb2020-06-23

ID:
10666114

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