GSM4474555: Cow: 4319 Trt: 63; Bos taurus; RNA-Seq - SRA

SRX8111198: GSM4474555: Cow: 4319 Trt: 63; Bos taurus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 112.8M spots, 33G bases, 9.7Gb downloads

Submitted by: NCBI (GEO)

Study: Secreted milk transcriptome from early lactation dairy cows

PRJNA625085 • SRP256295 • All experiments • All runs

show Abstracthide Abstract

Our objective was to characterize the protein coding portion of the bovine mammary gland transcriptome by magnitude of RNA-seq read counts. EBseq analysis determined no differentially expressed genes due to postruminal lysine infusion, therefore, read counts were averaged across treatment and block. DAVID functional annotation analysis was utilized to determine specific gene ontologies and KEGG pathways to describe the data by magnitude of reads. Overall design: Six Holstein cows' milk RNA sequenced to determine effects of postruminal lysine treatment on mammary transcriptome profile.

Sample: Cow: 4319 Trt: 63

SAMN14592559 • SRS6476272 • All experiments • All runs

Organism: Bos taurus

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Milk samples were collected and centrifuged at 2,000 x g for 10 min to separate the milk fat. The milk fat was removed to a separate vial, an equal volume of RNAse free water and Trizol added. An equal volume of chloroform was added and the entire sample was centrifuged at 12,000 x g. After centrifuging 70% ethanol was added. RNA was cleaned by Qiagen RNAeasy Mini Kit. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM4474555

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 112.8M spots, 33G bases, 9.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR11540896	112,822,800	33G	9.7Gb	2021-03-21

ID:: 10561064

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