GSM4456070: control rep2; Caulobacter vibrioides; RIP-Seq - SRA

SRX8073075: GSM4456070: control rep2; Caulobacter vibrioides; RIP-Seq
1 ILLUMINA (Illumina MiSeq) run: 3.9M spots, 580.3M bases, 330.7Mb downloads

Submitted by: NCBI (GEO)

Study: RNA co-immunoprecipitation with 3xFLAG::Hfq in Caulobacter crescentus

PRJNA623516 • SRP255571 • All experiments • All runs

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In this study we used RNA co-immunoprecipitation followed by RNA-sequencing to identify Hfq-binding RNAs in C. crescentus. Overall design: C. crescentus wild-type and 3xFLAG::hfq strains were cultivated in PYE medium to OD660 of 1.0. Cells were subjected to RNA co-immunoprecipitation and RNA was isolated. The experiment was performed with two independent biological replicates.

Sample: control rep2

SAMN14548594 • SRS6441561 • All experiments • All runs

Organism: Caulobacter vibrioides NA1000

Library:

Instrument: Illumina MiSeq

Strategy: RIP-Seq

Source: TRANSCRIPTOMIC

Selection: other

Layout: SINGLE

Construction protocol: RNA was isolated by phenol-chloroform extraction using standard protocols. Prepared RNA was digested with DNaseI. Integrity of the prepared RNA was tested using a Bioanalyzer. cDNA libraries were prepared using the NEBNext Small RNA Library Prep Set for Illumina (NEB E7300), according to the manufacturer's instructions. Quality of the prepared libraries was tested using a Bioanalyzer.

Experiment attributes:

GEO Accession: GSM4456070

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 3.9M spots, 580.3M bases, 330.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR11497152	3,872,574	580.3M	330.7Mb	2023-01-01

ID:: 10515527

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