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SRX8036800: GSM4446863: miRNA_12_IL1_a; Mus musculus; miRNA-Seq
2 ILLUMINA (Illumina HiSeq 3000) runs: 17M spots, 848.2M bases, 299.2Mb downloads

Submitted by: NCBI (GEO)
Study: The miRNA-mRNA Interactome of murine iPSC-derived Chondrocytes in Response to Inflammatory Cytokines
show Abstracthide Abstract
The goal of this study was to understand the mechanisms of miRNA regulation of the transcriptome of tissue engineered cartilage in response to IL-1ß and TNF-a using an in vitro murine induced pluripotent stem cell (miPSC) model system. miPSCs were chondrogenically differentiated for 21 days and then treated with 1 ng/mL of IL-1ß, 20 ng/mL of TNF-a, or control media without cytokines for 0, 4, 12, 24, and 72 hours. We performed an integrative analysis of miRNA- and RNA-sequencing data to determine the temporal and dynamic responses of miRNAs and genes to specific inflammatory cytokines. Through integration of mRNA and miRNA sequencing data, we created networks of miRNA-mRNA interactions which may be controlling the response to inflammatory cytokines. Overall design: miRNA and mRNA profiles of tissue-engineered cartilage; in triplicate, using a Illumina HiSeq3000
Sample: miRNA_12_IL1_a
SAMN14502017 • SRS6409036 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 3000
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Frozen tissue-engineered cartilage samples were homogenized with a miniature bead beater. Total cellular RNA was isolated using the mirVana miRNA Isolation Kit (Invitrogen) for small RNA sequencing or Total RNA Purification Plus Kit (Norgen) for mRNA sequencing. miRNA: Illumina TreSeq Small RNA kit; mRNA: Illumina Ribo-Zero Gold kit
Experiment attributes:
GEO Accession: GSM4446863
Links:
Runs: 2 runs, 17M spots, 848.2M bases, 299.2Mb
Run# of Spots# of BasesSizePublished
SRR114593359,040,087452M147.4Mb2020-08-12
SRR114593367,924,326396.2M151.8Mb2020-08-12

ID:
10478539

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