GSM1561400: HPM3-A1; Homo sapiens; RNA-Seq - SRA

SRX800033: GSM1561400: HPM3-A1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 1000) run: 37.3M spots, 7.5G bases, 4.9Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Differential susceptibility of human pleural and peritoneal mesothelial cells to asbestos exposure

PRJNA269624 • SRP050954 • All experiments • All runs

show Abstracthide Abstract

We hypothesize that the observed differences in incidences of pleural and peritoneal malignant mesothelioma (MM) are the result of differences in the direct response of these cell types to asbestos rather than to differences mediated by the in vivo microenvironment. To test this hypothesis, we characterized cellular responses to asbestos in a controlled environment using high-throughput RNA sequence and other assays. Overall design: Examination of asbestos-treated versus untreated mesothelial cells from four cell lines representing two tissue types in culture.

Sample: HPM3-A1

SAMN03255841 • SRS783001 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 1000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: 1 μg of total RNA was subjected to library synthesis using the TruSeq V2 RNA-Seq kit. 1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes prior to 12 cycles of PCR. Sequencing was performed using 12pM hybridization to a 2x100 paired end flow cell.

Experiment attributes:

GEO Accession: GSM1561400

Links:

External link: GEO Sample GSM1561400

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 37.3M spots, 7.5G bases, 4.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR1697337	37,333,445	7.5G	4.9Gb	2015-03-02

ID:: 1143609

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Sequence Read Archive

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