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SRX7973261: GSM4430241: LPM_DMSO_Rep1; Salmonella enterica subsp. enterica serovar Typhimurium; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 2.5M spots, 498.7M bases, 257.5Mb downloads

Submitted by: NCBI (GEO)
Study: RNA-sequencing of dephostatin-treated Salmonella
PRJNA614513 • SRP253755 • All experiments • All runs
show Abstracthide Abstract
We report the effect of dephostatin treatment on the Salmonella transcriptome. High-throughput sequencing was used to quantify transcript levels under different growth conditions. Overall design: Salmonella was grown in the presence of dephostatin and DMSO in SPI-2 inducing (LPM) and non-inducing (LB) media types. RNA was isolated and sequenced to quantify differential gene expression induced by dephostatin treatment.
Sample: LPM_DMSO_Rep1
SAMN14428296 • SRS6358059 • All experiments • All runs
Organism: Salmonella enterica subsp. enterica serovar Typhimurium
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted with Trizol-chloroform separation, precipitated with isopropanol, washed with 75% ethanol, then treated with Dnase I. Purified total RNA was treated with RiboZero for rRNA depletion and strand-specific dUTP sequencing was performed on an Illumina HiSeq 2000 platform to obtain single-end reads.
Experiment attributes:
GEO Accession: GSM4430241
Links:
Runs: 1 run, 2.5M spots, 498.7M bases, 257.5Mb
Run# of Spots# of BasesSizePublished
SRR113944442,481,138498.7M257.5Mb2020-03-25

ID:
10409087

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