GSM4408752: 24H10Z_3; Homo sapiens; RNA-Seq - SRA

SRX7901495: GSM4408752: 24H10Z_3; Homo sapiens; RNA-Seq
1 ION_TORRENT (Ion Torrent Proton) run: 3.7M spots, 524.7M bases, 312.2Mb downloads

Submitted by: NCBI (GEO)

Study: An in vitro model of chronic wounding and its implication for age-related macular degeneration

PRJNA612295 • SRP252574 • All experiments • All runs

show Abstracthide Abstract

Age-related macular degeneration (AMD) is a leading cause of blindness in the developed world. In this study we investigated changes in differentated human retinal pigmented epithelial (RPE) cells to acute compared to chronic wounding. There are many interesting phenotypic and transcriptomic changes that occur after chronic wounding compared to acute wounding conditions which may have implications for AMD. Overall design: Examination of mRNA expression in acutely or chronicaly wounded human RPE cultures 5-hours, 24-hours and 8-days after final wound. Unwounded cultures served as controls.

Sample: 24H10Z_3

SAMN14366121 • SRS6309232 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Ion Torrent Proton

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was harvested using NucleoSpin RNA XS kit (Clontech Laboratories) RNA was converted into cDNA using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories). DNA libraries were prepared with Ion Xpress™ Plus gDNA Fragment Library Preparation kit and sequenced by an Ion Proton next-generation sequencer (Thermo Fisher Scientific Inc.).

Experiment attributes:

GEO Accession: GSM4408752

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 3.7M spots, 524.7M bases, 312.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR11296094	3,734,629	524.7M	312.2Mb	2020-07-08

ID:: 10336122

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity