Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted from snap-frozen xenografts chilled under liquid nitrogen to prevent thawing. RNA was extracted with TRIzol reagent and purified on an RNeasy mini column (Qiagen). On-column DNase digestion was performed with Qiagen RNase-free DNase kit. RNA-seq library preparation by following the Illumina TruSeq stranded mRNA sample preparation guide