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SRX7784835: GSM4332381: W9_0.01_8hr_Rep3; Rousettus aegyptiacus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 6.5M spots, 651.9M bases, 267.6Mb downloads

Submitted by: NCBI (GEO)
Study: Different Egyptian rousette interferon omega proteins elicit an overlapping but not identical transcriptional response in bat cells.
show Abstracthide Abstract
Abstract from accompanying publication: "Bats host a number of viruses that cause severe disease in humans without experiencing overt symptoms of disease themselves. While the mechanisms underlying this ability to avoid sickness are not known, deep sequencing studies of bat genomes have uncovered genetic adaptations that may have functional importance in the antiviral response of these animals. Egyptian rousette bats (Rousettus aegyptiacus) are the natural reservoir hosts of Marburg virus (MARV). In contrast to humans, these bats do not become sick when infected with MARV. A striking difference to the human genome is that Egyptian rousettes have an expanded repertoire of IFNW genes. To probe the biological implications of this expansion, we synthesized IFN-?4 and IFN-?9 proteins and tested their antiviral activity in Egyptian rousette cells. Both IFN-?4 and IFN-?9 showed antiviral activity against RNA viruses, including MARV, with IFN-?9 being more efficient than IFN-?4. Using RNA-Seq, we examined the transcriptional response induced by each protein. Although the sets of genes induced by the two IFNs were largely overlapping, IFN-?9 induced a more rapid and intense response than did IFN-?4. About 13% of genes induced by IFN-? treatment are not found in the Interferome or other ISG databases, indicating that they may be uniquely IFN-responsive in this bat." Overall design: mRNA trancriptional profiles of bat cells pretreated with bat interferons, media, universal interferon, or an unrelated protein for 4 or 8 hours at three different concentrations, in triplicate, sequenced on Illumina HiSeq 2500
Sample: W9_0.01_8hr_Rep3
SAMN14167104 • SRS6200849 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was harvested in RNAzol RT and frozen at -80°C prior to extraction by pelleting out of DNA and proteins and then precipitation with isopropanol. RNA libraries were prepared with the Illumina TruSeq® Stranded mRNA Library Prep kit. Pooled libraries underwent pippin size selection.
Experiment attributes:
GEO Accession: GSM4332381
Links:
Runs: 1 run, 6.5M spots, 651.9M bases, 267.6Mb
Run# of Spots# of BasesSizePublished
SRR111487056,454,743651.9M267.6Mb2020-03-17

ID:
10163063

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