GSM4318346: LM sample 6; Homo sapiens; RNA-Seq - SRA

SRX7737611: GSM4318346: LM sample 6; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 63M spots, 5.4G bases, 2Gb downloads

Submitted by: NCBI (GEO)

Study: Reducing metastases stiffness by targeting fibroblasts improves therapeutic outcome in colorectal cancer [Tissue]

PRJNA607210 • SRP249792 • All experiments • All runs

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Analysis of colorectal primary tumors (pTU) and liver metastases (LM) tissue samples from patients and identify corresponding gene expression signatures Overall design: 12 pTU and 10 LM tissue samples deriving from 22 patients are examinated with Next Generation Sequencing(NGS)

Sample: LM sample 6

SAMN14124293 • SRS6157685 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Tumor tissues were removed, flash frozen on dry ice, and RNA was harvested using the mini RNA extraction kit (Qiagen, Germany; 74106). Sequencing was performed by the GeneCore at the EMBL (Heidelberg, Germany) on an Illumina HiSeq 2000 platform using the Truseq stranded mRNA kit from Illumina. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM4318346

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 63M spots, 5.4G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR11098966	63,044,530	5.4G	2Gb	2020-05-05

ID:: 10115624

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