GSM4300228: DDB_G0281091- 4h; Dictyostelium discoideum; RNA-Seq - SRA

SRX7691910: GSM4300228: DDB_G0281091- 4h; Dictyostelium discoideum; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 5.2M spots, 788.7M bases, 294.7Mb downloads

Submitted by: NCBI (GEO)

Study: Cell and molecular transitions during efficient dedifferentiation: testing transcription factor mutants

PRJNA605213 • SRP247605 • All experiments • All runs

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Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type with bzpS-, mybD-, nfyA-, DDB_G0281091-, DDB_G0269374- and DDB_G0272386- mutant cells dedifferentiating in growth medium Overall design: RNASeq timecourses (0,2,4,6,8,24 h) of dedifferentation in growth media for wild type (AX2), bzpS-, mybD-, nfyA-, DDB_G0281091-, DDB_G0269374-, DDB_G0272386- cells. N = 1

Sample: DDB_G0281091- 4h

SAMN14052332 • SRS6118036 • All experiments • All runs

Organism: Dictyostelium discoideum

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were pelleted and flash frozen. Total RNA was extracted by SDS-based lysis, followed by phenol extraction and ethanol preciptiation to remove protein. 100 ng total RNA was used to prepare mRNA libraries using NEBNext mRNA isolation kit and NEBNext Ultra II Directional RNA Library preparation kit. mRNA fragmentation used manufacturer recommended conditions for a 300bp insert size. Library amplification used 13 cycles of PCR. 75bp paired-end reads were generated using an Illumina NextSeq 500.

Experiment attributes:

GEO Accession: GSM4300228

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 5.2M spots, 788.7M bases, 294.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR11039985	5,226,456	788.7M	294.7Mb	2020-04-10

ID:: 10040131

SRA

Sequence Read Archive

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