GSM4300097: Media 4h rep1; Dictyostelium discoideum; RNA-Seq - SRA

SRX7691772: GSM4300097: Media 4h rep1; Dictyostelium discoideum; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 13.4M spots, 2G bases, 731.7Mb downloads

Submitted by: NCBI (GEO)

Study: Cell and molecular transitions during efficient dedifferentiation: population timecourses

PRJNA605216 • SRP247602 • All experiments • All runs

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Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum, with comparison to forward development Overall design: RNAseq timecourses sampling the dedifferentiation of 14h developed cells in two different nutrient conditions; liquid growth media (Media, 0 to 18 h) and Klebsiella bacteria (Bacteria, 0 to 24 h), compared with mock dedifferentiation in phosphate buffer (Buffer, 0 to 6 h), a reference sample of undifferentiated cells (Undifferentiated) and a timecourse of forward development (Development, 0 to 14 h). All timecourses performed in duplicate.

Sample: Media 4h rep1

SAMN14052223 • SRS6117904 • All experiments • All runs

Organism: Dictyostelium discoideum

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were pelleted and flash frozen. Total RNA was extracted by SDS-based lysis, followed by phenol extraction and ethanol preciptiation to remove protein. 100 ng total RNA was used to prepare mRNA libraries using NEBNext mRNA isolation kit and NEBNext Ultra Directional RNA Library preparation kit. mRNA fragmentation used manufacturer recommended conditions for a 300bp insert size. Library amplification used 13 cycles of PCR. 75bp paired-end reads were generated using an Illumina NextSeq 500.

Experiment attributes:

GEO Accession: GSM4300097

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 13.4M spots, 2G bases, 731.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR11039850	13,356,818	2G	731.7Mb	2020-04-10

ID:: 10039993

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