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SRX7652765: GSM4290901: locPDAC06; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 4M spots, 564.9M bases, 208.3Mb downloads

Submitted by: NCBI (GEO)
Study: Pancreatic Circulating Tumour Cell Profiling Identifies LIN28B as a Metastasis Driver and Drug Target
PRJNA604022 • SRP246180 • All experiments • All runs
show Abstracthide Abstract
Gene expression profiling of circulating tumor cells purified from human patients with pancreatic cancer led to identification of LIN28B as possible metastasis driver. Functional studies in model systems confirmed the importance of this pathway. Overall design: RNA-seq of primary purified circulating tumor cells from pancreatic cancer patients and healthy donors.
Sample: locPDAC06
SAMN13948117 • SRS6082225 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA isolation was performed using the AllPrep kit (Qiagen) including the optional on column DNAse treatment (Qiagen) per manufacturer's protocol. To generate libraries for total RNA sequencing the Illumina Smarter Stranded Total RNA-Seq kit v2 (Cat No#634413) was used according to the manufacturer's instructions. Pooled libraries were sequenced on an Illumina NextSeq 500 sequencer.
Experiment attributes:
GEO Accession: GSM4290901
Links:
Runs: 1 run, 4M spots, 564.9M bases, 208.3Mb
Run# of Spots# of BasesSizePublished
SRR109914823,966,987564.9M208.3Mb2020-03-02

ID:
9983854

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