Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was isolated using hot borate (Wan and Wilkins, 1994) followed by column-purification (Zymo Research, Irvine, CA, USA), and mRNA amplified (one-round) with TargetAmp Amplification Kit (Epicenter, Madison, WI, USA). Amplified mRNA was quantified by bioanalyzer (Agilent, Santa Clara, CA, USA), and 125 ng used to prepare Illumina TruSeq mRNA stranded libraries (Illumina, Inc., San Diego, CA, USA). 125 ng of amplified mRNA was used to prepare Illumina TruSeq mRNA stranded libraries (Illumina, Inc., San Diego, CA, USA).