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SRX7622735: GSM4280423: WT_Rep2; Oryza sativa Indica Group; ncRNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 21.4M spots, 1.1G bases, 498.7Mb downloads

Submitted by: NCBI (GEO)
Study: Role of rice AGO17 in rice development
show Abstracthide Abstract
Artificial miRNA mediated knock-down ago17 transgenic lines showed developmentally compromised phenotype in both vegetative and reproductive tissues compared to wild-type control, whereas the overexpression of AGO17 enhanced plant growth and yield. We have performed small RNA seq to investigate underlying molecular mechanism and to reveal functional role of AGO17 in rice. Overall design: We have performed small RNA-seq analysis using mature panicles (Stage 12) of WT, over-expression and knock-down transgenic lines of AGO17. Libraries were prepared from total RNA isolated from the tissues mentioned.
Sample: WT_Rep2
SAMN13908206 • SRS6054689 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: ncRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total RNA mature panicle before anthesis (Stage 12) of both wild-type, over-expression and transgenic knock-down lines of AGO17 were extracted using Trizol (Invitrogen) reagent. Size fractionation
Experiment attributes:
GEO Accession: GSM4280423
Links:
Runs: 1 run, 21.4M spots, 1.1G bases, 498.7Mb
Run# of Spots# of BasesSizePublished
SRR1095650221,425,3541.1G498.7Mb2020-10-02

ID:
9950865

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