GSM4279330: CD-T3A_S175; Nematostella vectensis; RNA-Seq - SRA

SRX7619000: GSM4279330: CD-T3A_S175; Nematostella vectensis; RNA-Seq
6 ILLUMINA (Illumina HiSeq 2500) runs: 4.3M spots, 435.8M bases, 151.1Mb downloads

Submitted by: NCBI (GEO)

Study: Gene expression during recovery from different forms of trauma in the sea anemone, Nematostella vectensis

PRJNA602703 • SRP243928 • All experiments • All runs

show Abstracthide Abstract

We exposed N. vectensis to three forms of trauma (streptomycin, cytochalasin D, or calcium-free seawater) to determine differences in gene expression during recovery from the trauma. Gene expression was monitored during different time points of recovery for animals exposed to cytochalasin D or calcium-free seawater. Gene expression was monitored following designated times of exposure and after 1 hour of recovery for animals exposed to streptomycin. Overall design: total 84 samples: 14 time points (3 replicates each) for 2 conditions. (14*3*2 = 84)

Sample: CD-T3A_S175

SAMN13901821 • SRS6051376 • All experiments • All runs

Organism: Nematostella vectensis

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted from oral discs and tentacles (flash frozen in liquid nitrogen/stored at -80) using Ambion RNAqueous extraction kit. Libraries were prepared as in Meyer et al. (2011)

Experiment attributes:

GEO Accession: GSM4279330

Links:

NCBI link: NCBI Entrez (gds)

Runs: 6 runs, 4.3M spots, 435.8M bases, 151.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR10952294	794,963	80.3M	27.4Mb	2020-01-23
SRR10952295	684,485	69.1M	23.4Mb	2020-01-23
SRR10952296	714,879	72.2M	26.1Mb	2020-01-23
SRR10952297	716,661	72.4M	25.6Mb	2020-01-23
SRR10952298	690,081	69.7M	24.9Mb	2020-01-23
SRR10952299	713,626	72.1M	23.8Mb	2020-01-23

ID:: 9944659

SRA

Sequence Read Archive

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