GSM4271192: Ovaries, L2; Anas platyrhynchos; RNA-Seq - SRA

SRX7554596: GSM4271192: Ovaries, L2; Anas platyrhynchos; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 26.4M spots, 7.9G bases, 3Gb downloads

Submitted by: NCBI (GEO)

Study: Regulation of actin cytoskeleton signaling pathways improves egg-production through up-regulation of integrin beta family genes expression in ducks

PRJNA601181 • SRP241926 • All experiments • All runs

show Abstracthide Abstract

Ovaries transcriptomic profiling between of egg of three high number of laying eggs (HEN) and three low number of laying eggs (LEN) in Longyan Shan-ma duck at 71 weeks were sequenced using Illumina Hiseq 2500 technology. Overall design: At 71 weeks, three duck with the high egg number (HEN) and three duck with the low egg number (LEN) from full-sib family were weighed and the number of laying egg, average egg weight and total egg weight were calculated. The ducks were killed by stunning and exsanguination, 12 h after feed was withheld. Samples from the ovarian tissue were separated and snap-frozen in liquid nitrogen until RNA sequencing and analysis of relative mRNA expression

Sample: Ovaries, L2

SAMN13853741 • SRS5991997 • All experiments • All runs

Organism: Anas platyrhynchos

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: ovarian tissue were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. TRIzol RNA Isolation Reagents was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM4271192

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 26.4M spots, 7.9G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
SRR10885615	26,393,969	7.9G	3Gb	2023-01-01

ID:: 9869271

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity