GSM4263505: SCC10-PDX1; Homo sapiens; RNA-Seq - SRA

SRX7551069: GSM4263505: SCC10-PDX1; Homo sapiens; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 51.5M spots, 5.2G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)

Study: Characterization of the mechansims involved in resistance to EGFR inhibitors

PRJNA600989 • SRP241820 • All experiments • All runs

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We generated patient cSCC-derived xenografts (cSCC-PDXs) that recapitulate the histopathologic and molecular features of parental patient samples. Response to gefitinib treatment was tested in four independent models. cSCC34-PDXs showed intrinsic resistance to gefitinib, whereas three models (cSCC10-PDXs, cSCC16-PDXs and cSCC24-PDXs) initially showed a strong response to gefitinib, but acquired resistance after long-term treatment. RNA sequencing analysis were performed in matched untreated and gefitinib-resistant cSCC-PDXs to determine mechanisms behind gefitinib resistance. Overall design: RNA sequencing of control (treated with vehicle solution) cSCC-PDXs vs gefitinib-resistant cSCC-PDXs from four different patients

Sample: SCC10-PDX1

SAMN13839613 • SRS5988578 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was isolated from FACS-sorted tumor cells by using RNAesay Mini Kit (Qiagen). RNA quality was assessed by Agilent 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina TruSeq protocols

Experiment attributes:

GEO Accession: GSM4263505

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 51.5M spots, 5.2G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR10881987	16,267,240	1.6G	777.9Mb	2023-01-12
SRR10881988	35,276,391	3.5G	1.7Gb	2023-01-12

ID:: 9865142

SRA

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