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SRX7426544: GSM4228114: W081_young_leaf_RNAPII_rep2; Oryza sativa; ChIP-Seq
1 ILLUMINA (HiSeq X Ten) run: 15.4M spots, 4.6G bases, 1.8Gb downloads

Submitted by: NCBI (GEO)
Study: Integrative analysis of reference epigenomes in rice [ChIP-seq]
show Abstracthide Abstract
Approximately 73% of rice genomes were annotated with different epigenomic properties. Refinement of promoter regions using open chromatin and H3K4me3-marked regions provided insight into transcriptional regulation. Active and repressed histone modifications and the predicted enhancers varied largely across tissues, whereas inactive chromatin states were relatively stable. Further, we investigated the impact of genetic variants on epigenomic signals and gene expression. Together, these datasets constitute a resource for functional element annotation in rice and indicate the central role of epigenomic information in understanding transcriptional regulation. Overall design: Here, we generated genome-wide profiling of five histone modifications and RNA polymerase II occupancy using an enhanced chromatin immunoprecipitation (eChIP) approach. By integrating chromatin accessibility, DNA methylation, and transcriptome datasets, we constructed comprehensive epigenome landscapes across various tissues
Sample: W081_young_leaf_RNAPII_rep2
SAMN13662611 • SRS5872644 • All experiments • All runs
Organism: Oryza sativa
Library:
Instrument: HiSeq X Ten
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-DNA was extracted with phenol:chloroform:isoamyl alcohol (Sigma–Aldrich, P3803), precipitated with ethanol, and resuspended in TE buffer. ChIP DNA libraries were prepared using an NEBNext® Ultra™ II DNA library prep kit for Illumina® (New England BioLabs, E7645). Briefly, ChIP DNA was end-repaired, ligated with an adaptor, and followed by 6–10 cycles of PCR amplification, per the manufacturer's guidelines. Next, library fragments of 250–650 bp were selected using AMPure XP beads (Beckman, A63881). Finally, the DNA fragments were sequenced using an Illumina HiSeq X Ten system (paired-end 150-bp reads)
Experiment attributes:
GEO Accession: GSM4228114
Links:
Runs: 1 run, 15.4M spots, 4.6G bases, 1.8Gb
Run# of Spots# of BasesSizePublished
SRR1075186115,401,8004.6G1.8Gb2020-04-25

ID:
9718379

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