Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: For ATAC-Seq, 50 000 cells were lysed and treated with Tn Transposase, then purified using MinElute PCR purification kit (Qiagen). For ChIP-Seq, histone-DNA complexes were isolated from sonicated nuclei using bead-immobilized antibody. DNA was isolated by phenol/chloroform extraction. For RNAseq, cells were lysed in Trizol, and total RNA was isolated using Qiagen columns. RNA was treated with DNAse once on-column and once in-tube using Turbo DNAse kit (Thermo FIsher). ATAC-Seq libraries were prepared using Nextera PCR primers (Illumina). ChIP-Seq libraries were prepared using TruSeq ChIP Libary Preparation Kit (Illumina). RNAseq libraries were prepared using KAPA Stranded RNA-Seq with RiboErase Kit (Roche/KAPA Biosystems)