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SRX7366923: GSM4217490: IRBB5_24h_1; Oryza sativa Japonica Group; miRNA-Seq
1 BGISEQ (BGISEQ-500) run: 27.7M spots, 636.4M bases, 423.1Mb downloads

Submitted by: NCBI (GEO)
Study: Characteristic dissection of Xanthomonas oryzae pv. oryzae responsive microRNAs in rice [datatset 2]
show Abstracthide Abstract
we emphatically monitored the responsive changes of rice miRNAs at 0, 8, 24 hours across Xoo strain PXO86 infection in its compatible rice variety IR24 and incompatible variety IRBB5 by small RNA sequencing, and the genes targeted by miRNAs were also detected via degradome technology. These findings provide new insights into the complex roles of characteristic miRNAs and their targets in rice-Xoo interactions. Overall design: Examination of the responsive changes of rice miRNAs and their targets at 0, 8, 24 hours across Xoo strain PXO86 infection in its compatible rice variety IR24 and incompatible variety IRBB5 by small RNA sequencing and degradome technology
Sample: IRBB5_24h_1
SAMN13558925 • SRS5825405 • All experiments • All runs
Library:
Instrument: BGISEQ-500
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Every sample was pooled with infected leaves collected from twenty plants in one plot to diminish the individual environment differences.Total RNA were extracted from the mentioned samples using Trizol reagent (Invitrogen, USA) according to the manufacturer's recommendation. Small RNAs with 18~30 nt in length were isolated from the total RNA using a polyacrylamide gel electrophoresis gel, and then were sequenced by the BGISEQ-500.
Experiment attributes:
GEO Accession: GSM4217490
Links:
Runs: 1 run, 27.7M spots, 636.4M bases, 423.1Mb
Run# of Spots# of BasesSizePublished
SRR1069015727,691,057636.4M423.1Mb2020-03-16

ID:
9651754

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