GSM4101389: MALA3; Mus musculus; RNA-Seq - SRA

SRX6923960: GSM4101389: MALA3; Mus musculus; RNA-Seq
4 ILLUMINA (NextSeq 500) runs: 20.5M spots, 1.7G bases, 710.4Mb downloads

Submitted by: NCBI (GEO)

Study: Microbes Malnutrition Microglia

PRJNA575001 • SRP223737 • All experiments • All runs

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This study examines the role of early exposure to gut microbes and poor diet on microglial function in mice. Groups = control (CON), malnourished (MAL), and malnourished + microbial exposure (E/MALBG). CD11b+ cells (microglial enrichment) were isolated from whole mouse brains (Adult Brain Disruption Kit, Miltenyi Biotec). After sample quality control (Agilent 2100 Bioanalyzer), qualifying samples were sent for RNA-Seq (Illumina NextSeq 500 with Paired End 42bp × 42bp reads; demultiplexed: Illumina's bcl2fastq2). Following alignment against mouse reference genes (STAR aligner), DEG analyses was conducted using the DESeq2 pipeline. Overall design: RNA-Seq from Cd11b+ brain cells

Sample: MALA3

SAMN12873973 • SRS5454842 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cortical brain tissue was harvested and stored in RPMI media on ice before cell isolation and CD11b+ enrichment using Miltenyi Biotec murine kits Standard Illumina protocols prepared by BRC-Seq (Sequencing core at the University of British Columbia, Vancouver, Canada)

Experiment attributes:

GEO Accession: GSM4101389

Links:

NCBI link: NCBI Entrez (gds)

Runs: 4 runs, 20.5M spots, 1.7G bases, 710.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR10203933	5,162,245	439M	178.3Mb	2020-04-01
SRR10203934	5,071,800	431.3M	175Mb	2020-04-01
SRR10203935	5,142,758	437.3M	180.1Mb	2020-04-01
SRR10203936	5,076,250	431.7M	176.9Mb	2020-04-01

ID:: 9106737

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