Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: total RNA from homogenized brain tissue was extracted using RNeasy Micro (QIAGEN, Hilden, Germany) following manufacturer’s instructions. Library preparation for RNA-Seq was performed using the TruSeq Stranded Total RNA with Ribo-Zero Gold removing both cytoplasmic and mitochondrial rRNA (Illumina, Cat N° RS-122-2201). 200 ng of total RNA were used as start material . Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega). The size range of final cDNA libraries was determined applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). cDNA libraries were amplified and sequenced by using the cBot and HiSeq2000 from Illumina (SR; 1x50 bp;5- 6 GB ca. 30-35 million reads per sample).