Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted using the RNeasy protect mini kit (Qiagen), while the DNA present was removed with RNase-Free DNase Set (Qiagen). For the preparation of sequencing library, ribosomal RNA was removed from 1 µg of RNA per sample, using the Illumina Ribo-zero rRNA removal kit, and purified with an Agencourt RNAClean XP kit. Indexed libraries were prepared using the ScriptSeqTM v2 RNA-Seq Library Preparation Kit and ScriptSeqTM Index PCR Primers-Set 1 (Illumina). The library size was profiled with the Bioanalyzer High Sensitivity Assay Kit (Agilent) and quantified with the Qubit HS DNA Assay Kit. The samples were diluted and spiked with a Phix control library (Illumina).