GSM1477388: aFLe2_mRNAseq; Anolis carolinensis; RNA-Seq - SRA

SRX679302: GSM1477388: aFLe2_mRNAseq; Anolis carolinensis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 20.3M spots, 1G bases, 617.5Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: A relative shift in cloacal location repositions external genitalia in amniote evolution

PRJNA258129 • SRP045471 • All experiments • All runs

show Abstracthide Abstract

Comparative RNA-seq profiling of mouse and anole lizard developing limbs and external genitalia, to assess evolutionary and develomental relationships, between the two tissue types based on transcriptomic data Overall design: RNA-seq profiling of embryonic limb and external genitalia tissue at different stages of development, in mouse and anole lizard, in duplicates, using Illumina HiSeq

Sample: aFLe2_mRNAseq

SAMN02988839 • SRS682459 • All experiments • All runs

Organism: Anolis carolinensis

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted using Arcturus PicoPure RNA Isolation Kit (Life Technologies) and enriched for mRNA fraction using MPG mRNA Purification Kit (PureBiotech) Multiplexed RNA-seq libraries were produced using the SPIA cDNA synthesis kit and the Ovation Ultralow DR Multiplex system (NuGen)

Experiment attributes:

GEO Accession: GSM1477388

Links:

External link: GEO Sample GSM1477388

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 20.3M spots, 1G bases, 617.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR1548570	20,288,993	1G	617.5Mb	2014-08-15

ID:: 951312

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