GSM4044996: KO3; Mycobacterium tuberculosis H37Ra; RNA-Seq - SRA

SRX6759377: GSM4044996: KO3; Mycobacterium tuberculosis H37Ra; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 11.1M spots, 3.3G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)

Study: Genome-wide maps of gene transcription level in wild-type and mpbR-deleted Mycobacterium tuberculosis H37Ra strains

PRJNA562277 • SRP219299 • All experiments • All runs

show Abstracthide Abstract

We report the application of RNA-seq technology for high-throughput profiling of gene transcription in M. tuberculosis H37Ra strains. By obtaining over four billion bases of sequence from mRNA, we generated genome-wide gene transcription maps of wild-type and mpbR-deleted Mycobacterium tuberculosis H37Ra strains. We find that a group of genes is significantly up-regulated in the mpbR-deleted mutant strain. This finding indicates that MpbR negatively regulates gene expression. Overall design: Examination of gene expression level in 2 strains.

Sample: KO3

SAMN12637216 • SRS5308097 • All experiments • All runs

Organism: Mycobacterium tuberculosis H37Ra

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA extraction protocol not provided. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM4044996

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 11.1M spots, 3.3G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR10022316	11,094,046	3.3G	1.3Gb	2022-11-10

ID:: 8903815

SRA

Sequence Read Archive

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