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SRX6734125: GSM4038538: Skin containing hair follicle , B6; Capra hircus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 21.2M spots, 6.2G bases, 2Gb downloads

Submitted by: NCBI (GEO)
Study: Skin transcriptome profiles associated with black- and white-coated region in a crossbred goat
show Abstracthide Abstract
we compared the skin transcriptomes of the black- and white-coated region from the Boer and Macheng Black crossbred goat with black head and white body using the Illumina RNA-Seq method. Six cDNA libraries derived from skin samples of the white coat region (n = 3) and black coat region (n = 3) were constructed from three full-sib goats. On average, we obtained approximately 76.5 and 73.5 million reads for each skin sample of black coat and white coat, respectively, of which 75.39% and 76.05% reads were covered in the genome database. Our study provides insight into the transcriptional regulation of two distinct coat color that might serve as a key resource for understanding coat color pigmentation of goat. Overall design: Examination of 2 different coat region in a crossbred goat.
Sample: Skin containing hair follicle , B6
SAMN12600143 • SRS5285449 • All experiments • All runs
Organism: Capra hircus
Library:
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: A total amount of 5 μg RNA per sample was isolated from each individual sample using TRIzol reagent (Invitrogen, USA). Purity and quantity of total RNA were measured by using Nanodrop equipment. Ribosomal RNAs were depleted using Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM4038538
Links:
Runs: 1 run, 21.2M spots, 6.2G bases, 2Gb
Run# of Spots# of BasesSizePublished
SRR999511821,174,5436.2G2Gb2022-08-19

ID:
8873291

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