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SRX6662824: GSM4011048: rancRNA_18-M2_total mRNA_rep2; Saccharomyces cerevisiae; RNA-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 24M spots, 7G bases, 2.7Gb downloads

Submitted by: NCBI (GEO)
Study: mRNA seq of total and polysome associated mRNAs in three different conditions
PRJNA559001 • SRP217590 • All experiments • All runs
show Abstracthide Abstract
We report on how the presence or absence of a 18 nt long ribosome-bound RNA affects the translation in S. cerevisiae. We observe that translation of all mRNAs is downregulated when the RNA is present. Overall design: Analysis of total and ribosome-associated mRNA in trm10? cells electroporated with no RNA, rancRNA_18 or rancRNA_18-M2.
Sample: rancRNA_18-M2_total mRNA_rep2
SAMN12511312 • SRS5223072 • All experiments • All runs
Organism: Saccharomyces cerevisiae
Library:
Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Yeast spheroplasts were electroporated with or without RNA, recovered and harvested by centrifugation. Pellets were opened using a FastPrep. Lysates were cleared by centrifugation and total RNA was extracted by hot acid phenol extraction. Poly-A-Enrichment via oligo-dT-beads. PolyA enriched mRNAs were transformed into a cDNA library using the Illumina TruSeq stranded mRNA Kit. Library size was determined using Qbit.
Experiment attributes:
GEO Accession: GSM4011048
Links:
Runs: 1 run, 24M spots, 7G bases, 2.7Gb
Run# of Spots# of BasesSizePublished
SRR991343823,993,1817G2.7Gb2021-12-09

ID:
8785834

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