GSM4011039: no RNA_total mRNA_rep3; Saccharomyces cerevisiae; RNA-Seq - SRA

SRX6662815: GSM4011039: no RNA_total mRNA_rep3; Saccharomyces cerevisiae; RNA-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 20.4M spots, 5.9G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)

Study: mRNA seq of total and polysome associated mRNAs in three different conditions

PRJNA559001 • SRP217590 • All experiments • All runs

show Abstracthide Abstract

We report on how the presence or absence of a 18 nt long ribosome-bound RNA affects the translation in S. cerevisiae. We observe that translation of all mRNAs is downregulated when the RNA is present. Overall design: Analysis of total and ribosome-associated mRNA in trm10? cells electroporated with no RNA, rancRNA_18 or rancRNA_18-M2.

Sample: no RNA_total mRNA_rep3

SAMN12511326 • SRS5223063 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Instrument: Illumina HiSeq 3000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Yeast spheroplasts were electroporated with or without RNA, recovered and harvested by centrifugation. Pellets were opened using a FastPrep. Lysates were cleared by centrifugation and total RNA was extracted by hot acid phenol extraction. Poly-A-Enrichment via oligo-dT-beads. PolyA enriched mRNAs were transformed into a cDNA library using the Illumina TruSeq stranded mRNA Kit. Library size was determined using Qbit.

Experiment attributes:

GEO Accession: GSM4011039

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 20.4M spots, 5.9G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR9913429	20,419,877	5.9G	2.4Gb	2021-12-09

ID:: 8785825

SRA

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