GSM4007090: THP1.2000nM.GSK032.d4.n1 [S1-40]; Homo sapiens; RNA-Seq - SRA

SRX6657138: GSM4007090: THP1.2000nM.GSK032.d4.n1 [S1-40]; Homo sapiens; RNA-Seq
6 ILLUMINA (Illumina NovaSeq 6000) runs: 102.6M spots, 31G bases, 9.1Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-seq of DNMT1 inhibitor treated AML cell lines

PRJNA558691 • SRP217480 • All experiments • All runs

show Abstracthide Abstract

Genome wide demethylation by DNMT1 inhibitors GSK3685032 results in up-regulation of epigenetically-silenced genes. Overall design: Differential expression was perfomed for each cell line following DNMT1 inhibitor or vehicle treatment across 6 point dose titration and 6 day timecourse. Differentially epressed gene lists were compared across the dose and timecourse.

Sample: THP1.2000nM.GSK032.d4.n1 [S1-40]

SAMN12496466 • SRS5217599 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was extracted using Qiagen Rneasy Mini kit Libraries were prepared at the NYU Medical Center Genome Technology Center All 150 PE libraries were sequenced across 6 lanes for a total of 12 fastq samples per sample.

Experiment attributes:

GEO Accession: GSM4007090

Links:

NCBI link: NCBI Entrez (gds)

Runs: 6 runs, 102.6M spots, 31G bases, 9.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR9905633	18,127,511	5.5G	1.6Gb	2021-06-30
SRR9905634	18,579,564	5.6G	1.6Gb	2021-06-30
SRR9905635	18,273,050	5.5G	1.6Gb	2021-06-30
SRR9905636	18,926,944	5.7G	1.7Gb	2021-06-30
SRR9905637	14,357,975	4.3G	1.3Gb	2021-06-30
SRR9905638	14,355,071	4.3G	1.3Gb	2021-06-30

ID:: 8780067

SRA

Sequence Read Archive

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