GSM3935780: UPF1_isoform_1_Total_3; Homo sapiens; RIP-Seq - SRA

SRX6423008: GSM3935780: UPF1_isoform_1_Total_3; Homo sapiens; RIP-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 63.9M spots, 9.6G bases, 3.7Gb downloads

Submitted by: NCBI (GEO)

Study: UPF1 regulatory loop variant RNA-Seq and RIP-Seq

PRJNA553576 • SRP213944 • All experiments • All runs

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HEK-293 Trex cells containing stable tetracycline-inducible transgenes driving overexpression of CLIP-tagged UPF1 isoforms 1 and 2 or GFP were used for total RNA-seq. In addition, CLIP-UPF1 isoforms were affinity purified, and bound RNA was subjected to high-throughput sequencing. Overall design: Paired-end high-throughput RNA sequencing of Total RNA from cells expressing CLIP-tagged UPF1 isoform 1, UPF1 isoform 2, or GFP and RNA associated with affinity-purified CLIP UPF1 isoforms 1 and 2.

Sample: UPF1_isoform_1_Total_3

SAMN12239729 • SRS5079799 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 3000

Strategy: RIP-Seq

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Construction protocol: Qiagen RNeasy Mini RNA isolation kit One μg of total RNA was used per sample for library preparation. Ribosomal RNA was depleted from total RNA with the Ribo-Zero rRNA Removal Kit (Epicentre), and purified RNA was then converted to cDNA with the TruSeq RNA Library Preparation Kit (Illumina).

Experiment attributes:

GEO Accession: GSM3935780

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 63.9M spots, 9.6G bases, 3.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR9662133	63,918,018	9.6G	3.7Gb	2020-05-28

ID:: 8507573

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