Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells were grown on YPD at 30°C in exponential phase. The Cryptococcus cell preparation was spiked in with one tenth (OD/OD) of S. cerevisiae strain FY834 cells grown in YPD at 30°C in stationary phase. Total RNA was extracted with Trizol. For RNA-Seq, strand-specific, paired-end cDNA libraries were prepared from 10 μg of total RNA by polyA selection using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer's instructions. cDNA fragments of ~400 bp were purified from each library and confirmed for quality by Bioanalyzer (Agilent). Then, 100 bases were sequenced from both ends using an Illumina HiSeq2500 instrument according to the manufacturer's instructions (Illumina).