Instrument: Illumina HiSeq 4000
Strategy: ncRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Inflorescence small RNA was isolated with Trizol reagent (Invitrogen) and concentrated using the mirVana microRNA Isolation Kit (Ambion). For AGO1-IP samples, AGO1 was immunocprecipitated from inflorescence tissue using Agrisera anti-AGO1 primary antibody. RNA was isolated from AGO1-IP or mock-IP samples using TriZol reagent. Size-enriched small RNAs were prepared using the TruSeq Small RNA Library Preparation Kit (Illumina). Multiplexed libraries were sequenced on HiSeq2500 or HiSeq4000