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SRX6099366: GSM3900141: scr_LPS_S15428; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 41.5M spots, 2.1G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: A first exon termination checkpoint that preferentially suppresses extragenic transcription [RNASEQ1_BMDM_4sU]
PRJNA549950 • SRP202030 • All experiments • All runs
show Abstracthide Abstract
Wdr82 and Zc3h4 repress transcription at extragenic sites. Overall design: Transcriptome analysis to measure transcription in cells depleted of Wdr82, Zc3h4or other known termination factors.
Sample: scr_LPS_S15428
SAMN12100508 • SRS4999893 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using Trizol whereafter nascent 4sU-labelled RNA was extracted as described in Austenaa et al. 2015 . Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) starting from ca. 100-300 ng of nascent 4sU-labelled RNA, without ribosomal depletion.
Experiment attributes:
GEO Accession: GSM3900141
Links:
Runs: 1 run, 41.5M spots, 2.1G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR933285741,474,8392.1G1.3Gb2021-02-01

ID:
8153416

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