GSM3723016: DMSO C; Mycobacterium tuberculosis; RNA-Seq - SRA

SRX5688215: GSM3723016: DMSO C; Mycobacterium tuberculosis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 6.6M spots, 329.7M bases, 196.2Mb downloads

Submitted by: NCBI (GEO)

Study: RNA-sequencing of hypoxic Mycobacterium tuberculosis treated with C10

PRJNA532918 • SRP192623 • All experiments • All runs

show Abstracthide Abstract

We sought to identify which genes were dysregulated in hypoxic Mycobacterium tuberculosis upon treatment with C10. We cultured Mycobacterium tuberculosis in an air-tight vessel for 2 weeks in the presence of either DMSO or 50 µM C10, and used RNA-sequencing to compare transcriptional profiles. Overall design: Three condition experiment: bacteria were cultured in hypoxic conditions in the presence of DMSO or 50 µM C10. Aerobically growing M. tuberculosis in 7H9 medium was included as a control. Each group was performed with three biological replicates.

Sample: DMSO C

SAMN11419543 • SRS4628120 • All experiments • All runs

Organism: Mycobacterium tuberculosis str. Erdman = ATCC 35801

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Bacteria were harvested from the culture by centrifugation, resuspended in Trizol and lysed by bead beating. RNA was extracted by chloroform extraction, followed by isopropanol precipitation, and the extracted RNA was resuspended in water. RNA integrity and concentration was measured with an Agilent Bioanalyzer. RNA libraries were prepared using standard Illumina protocols.

Experiment attributes:

GEO Accession: GSM3723016

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 6.6M spots, 329.7M bases, 196.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR8902365	6,593,440	329.7M	196.2Mb	2019-04-26

ID:: 7663154

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