GSM3611224: III^M #1 male head replicate 1; Musca domestica; RNA-Seq - SRA

SRX5387787: GSM3611224: III^M #1 male head replicate 1; Musca domestica; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 50.9M spots, 3.8G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)

Study: Effects of naturally ocurring proto-Y chromosomes on house fly gene expression

PRJNA522991 • SRP186164 • All experiments • All runs

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We performed RNA-seq experiments to examine the effects of house fly proto-Y chromosomes on gene expression. Two different Y^M chromosomes were investigated, and two different III^M chromosomes were investigated. One of the Y^M genotypes also was also heterozygous for third chromosomes that do not carry a male-determining locus to evaluate the effect of third chromosomes on gene expression independent of being a proto-Y. Overall design: Four different genotypes were assayed. For each genotype, expression was measured in both head and abdomen. Three biological replicates of each genotype and tissue were assayed.

Sample: III^M #1 male head replicate 1

SAMN10962298 • SRS4376230 • All experiments • All runs

Organism: Musca domestica

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Individual flies were dissected for abdomen and head tissues, frozen at -80˚C, and RNA was extracted from the individual tissues with TRIzol Reagent and the Zymo Direct-zol kit following the manufacturer's protocol including DNA digestion steps. The Illumina TruSeq Stranded mRNA Sample Preparation Kit was used with 4 ug of total RNA for the construction of sequencing libraries.

Experiment attributes:

GEO Accession: GSM3611224

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 50.9M spots, 3.8G bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR8587248	50,861,349	3.8G	1.7Gb	2019-02-20

ID:: 7290321

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