GSM3609532: Bap1MO rep2; Xenopus laevis; RNA-Seq - SRA

SRX5381421: GSM3609532: Bap1MO rep2; Xenopus laevis; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 40.8M spots, 3.1G bases, 1.1Gb downloads

Submitted by: NCBI (GEO)

Study: Bap1 loss leads to decreased expression of lineage specific commitment genes, and increased expression of pluripotency genes [RNA-seq]

PRJNA522475 • SRP185947 • All experiments • All runs

show Abstracthide Abstract

We performed morpholino-mediated knockdown of Bap1 protein expression in Xenopus laevis developing embryos, and analyzed gene expression at stage 12. We find that inhibition of Bap1 leads to decrease in lineage specific commitment genes, and increased expression of pluripotency genes. Overall design: RNA profiles of stage 12 embryos injected with either Ctrl morpholino (CtrlMO) or mopholino targeting Bap1 (Bap1MO), in triplicate, using Illumina Nextseq500.

Sample: Bap1MO rep2

SAMN10941054 • SRS4370500 • All experiments • All runs

Organism: Xenopus laevis

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was isolated with Trizol/Chloroform, and yolk and pigment were further removed from samples using RNeasy MinElute Cleanup Kit Libraries were prepared using the NEBNext® Ultra™ kit

Experiment attributes:

GEO Accession: GSM3609532

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 40.8M spots, 3.1G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR8580701	40,803,284	3.1G	1.1Gb	2019-09-26

ID:: 7279508

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