GSM3574677: MP2_Male_15_days; Daphnia magna; RNA-Seq - SRA

SRX5278683: GSM3574677: MP2_Male_15_days; Daphnia magna; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 22.5M spots, 4.5G bases, 1.5Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-sequencing analysis of genetically identical female and male Daphnia magna with age

PRJNA516443 • SRP181187 • All experiments • All runs

show Abstracthide Abstract

Comparison of female and male Daphnia magna gene expression with age. The sexes in Daphnia magna are genetically identical. The aim of this study was to identify possible differences in gene expression between genders with age. Overall design: RNA was extracted from samples with approximately equivalent dry mass using RNeasy Micro Kit (Qiagen Ltd, UK). The RNA-seq libraries were prepared using the Illumina TruSeq standard mRNA kit and sequenced (2x100bp) using Illumina Hi-Seq 4000 platform at BGI, Denmark.

Sample: MP2_Male_15_days

SAMN10788719 • SRS4278649 • All experiments • All runs

Organism: Daphnia magna

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted from the samples using RNeasy micro kit ((Qiagen Ltd, UK) according to the manufactures protocol. The RNA-seq libraries were prepared using the Illumina TruSeq standard mRNA kit and sequenced (2x100bp) using Illumina Hi-Seq 4000 platform at BGI, Denmark.

Experiment attributes:

GEO Accession: GSM3574677

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.5M spots, 4.5G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR8473164	22,510,393	4.5G	1.5Gb	2020-09-23

ID:: 7115469

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