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SRX5278211: GSM3574341: Control2; Rattus norvegicus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 38.6M spots, 5.8G bases, 2.2Gb downloads

Submitted by: NCBI (GEO)
Study: Diabetes severity is reflected by changes in a cardiac gene expression signature characterized by metabolism and cell death processes
show Abstracthide Abstract
We tested the impact of a graded reduction in insulin on cardiac gene expression with particular focus on metabolism and energy homeostasis. Wistar rats were made diabetic with a single dose of either 55 (D55) or 100 (D100) mg/kg streptozotocin (STZ). Although both D55 and D100 were equally hyperglycemic compared to control, D100 showed markedly lower plasma insulin and robust increases in plasma FA and TG. D100 demonstrated more significant transcriptomic changes than D55 with enrichment for metabolic processes that direct the heart to use FA, when glucose use is obstructed. Analysis of a protein-protein interaction network identified functional networks in D100 that describe mitochondrial overload, incomplete fatty acid oxidation, and loss of cardiomyocytes. Our data suggests that the differential reduction of insulin produced a dramatic change in cardiac gene expression largely enriched for substrate metabolism functions as well as a gene expression program supporting cell death. Overall design: We analyzed 4 control samples, 6 D55 samples and 6 D100 samples with RNA-Seq.
Sample: Control2
SAMN10788465 • SRS4278198 • All experiments • All runs
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Animals were followed for 4 days before termination at which time blood was collected and hearts exsanguinated. Total RNA from 4 control, 6 D55, and 6 D100 rats was isolated using TRIzol. Sequencing libraries were prepared from 400 ng total RNA using the TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA). Libraries were multiplexed and sequenced on the NextSeq 500 (Illumina).
Experiment attributes:
GEO Accession: GSM3574341
Links:
Runs: 1 run, 38.6M spots, 5.8G bases, 2.2Gb
Run# of Spots# of BasesSizePublished
SRR847265938,634,3165.8G2.2Gb2021-01-19

ID:
7114997

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    Activities or games, usually involving physical effort or skill. Reasons for engagement in sports include pleasure, competition, and/or financial reward....<br/>Year introduced: ATHLETICS was see under SPORTS 1963-1975
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