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SRX522636: GSM1371370: infected T474D 20 days rep 2; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 5.4M spots, 1.1G bases, 701.8Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Global variation of gene expression induced by constitutive activation of the NIK receptor in Arabidopsis
PRJNA244971 • SRP041308 • All experiments • All runs
show Abstracthide Abstract
Arabidopsis was transformed with the NIK receptor or the mutant T474D NIK receptor and infected with CaLCuV. The global variation of gene expression was analysed by RNA-seq at 10 days post inoculation and 20 days post inoculation and differential gene expression was determinated in comparison with mock inoculated Arabidopsis lines. Overall design: Pairwise comparison between mock inoculated and infected genotypes (WT,NIK-expressing lines and T474D-expressing lines) at 10 dpi and 20 dpi
Sample: infected T474D 20 days rep 2
SAMN02730145 • SRS594983 • All experiments • All runs
Organism: Arabidopsis thaliana
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: The transgenic and wild-type lines were infected at the seven-leaf stage with CaLCuV. After 10 days post-inoculation (dpi) or after 21 dpi, total RNA from systemically infected leaves, as diagnosed by PCR, and mock-inoculated leaves from wild-type, 35S::NIK1-5 and 35S::T474D-4 lines was isolated using TRIzol (Invitrogen) RNA pair-ended libraries were prepared by the Illumina HiSeq 2000 standard protocols
Experiment attributes:
GEO Accession: GSM1371370
Links:
External link:
Runs: 1 run, 5.4M spots, 1.1G bases, 701.8Mb
Run# of Spots# of BasesSizePublished
SRR12574085,358,2311.1G701.8Mb2014-04-21

ID:
722290

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