GSM1371368: mock T474D 20 days rep 2; Arabidopsis thaliana; RNA-Seq - SRA

SRX522634: GSM1371368: mock T474D 20 days rep 2; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 25.3M spots, 5.1G bases, 3.1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Global variation of gene expression induced by constitutive activation of the NIK receptor in Arabidopsis

PRJNA244971 • SRP041308 • All experiments • All runs

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Arabidopsis was transformed with the NIK receptor or the mutant T474D NIK receptor and infected with CaLCuV. The global variation of gene expression was analysed by RNA-seq at 10 days post inoculation and 20 days post inoculation and differential gene expression was determinated in comparison with mock inoculated Arabidopsis lines. Overall design: Pairwise comparison between mock inoculated and infected genotypes (WT,NIK-expressing lines and T474D-expressing lines) at 10 dpi and 20 dpi

Sample: mock T474D 20 days rep 2

SAMN02730144 • SRS594981 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: The transgenic and wild-type lines were infected at the seven-leaf stage with CaLCuV. After 10 days post-inoculation (dpi) or after 21 dpi, total RNA from systemically infected leaves, as diagnosed by PCR, and mock-inoculated leaves from wild-type, 35S::NIK1-5 and 35S::T474D-4 lines was isolated using TRIzol (Invitrogen) RNA pair-ended libraries were prepared by the Illumina HiSeq 2000 standard protocols

Experiment attributes:

GEO Accession: GSM1371368

Links:

External link: GEO Sample GSM1371368

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 25.3M spots, 5.1G bases, 3.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR1257406	25,283,948	5.1G	3.1Gb	2014-04-21

ID:: 722288

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