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SRX502869: GSM1359283: dog_piRNA_fraction; Canis lupus familiaris; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 65.9M spots, 3.3G bases, 1.8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transposable element targeting by piRNAs in Laurasiatherians with distinct TE activities
show Abstracthide Abstract
Transposable elements (TEs) are self-mobilizing elements that make up a large fraction of mammalian genomes. PIWI proteins and PIWI interacting RNAs (piRNAs) are a part of a system aimed at controlling and preventing TE proliferation. We examined the TE landscape and piRNA repertoires from three non-model Laurasiatherian mammals (dog, horse and big brown bat) with differing TE complements and proliferation patterns to address questions about the evolutionary relationships between piRNAs and TEs. We found that the genomic abundance of new TEs may not match TE transcription. We speculate that a higher rate of SINE targeting by piRNAs in the horse genome contributed to the reduced SINE insertion rate. Overall design: Two testicular tissue sample from three taxa. Six samples total
Sample: dog_piRNA_fraction
SAMN02711378 • SRS583675 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Testes were snap frozen from dog, horse, and a bat (Eptesicus fuscus). Total RNA was extracted from ground tissue using standard TRIzol protocols. The small RNA fraction was isolated and prepped for Illumina sequencing (50 bp, single-end).
Experiment attributes:
GEO Accession: GSM1359283
Links:
External link:
Runs: 1 run, 65.9M spots, 3.3G bases, 1.8Gb
Run# of Spots# of BasesSizePublished
SRR120731865,893,5403.3G1.8Gb2017-03-27

ID:
702119

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