GSM3439881: AK250_RydC_rep2_10min; Escherichia coli str. K-12 substr.... - SRA

SRX4912070: GSM3439881: AK250_RydC_rep2_10min; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 30.5M spots, 3G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)

Study: Next Generation Sequencing Facilitates Putative Target Identification for the Small RNA RydC

PRJNA497857 • SRP166315 • All experiments • All runs

show Abstracthide Abstract

We report the transcriptomic profile of Escherichia coli when the small RNA RydC is overexpressedand how this can be used to identify putative mRNA targets. This technique allowed us to identify potential mRNA targets to further validate in vivo. Overall design: We had two conditions: 1) No expression of the small RNA RydC and 2) Overexpression of the small RNA RydC

Sample: AK250_RydC_rep2_10min

SAMN10267405 • SRS3957661 • All experiments • All runs

Organism: Escherichia coli str. K-12 substr. MG1655

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted using the hot phenol method and ribosomal RNA removed using the Ribozero Bacteria kit (Illumina, CA) Strand-specific librares were prepared with Illumina's 'TruSeq Stranded RNA Sample Prep kit'; DNA libraries for RNA-Seq were prepared with the Library Construction kit from Kapa Biosystems

Experiment attributes:

GEO Accession: GSM3439881

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 30.5M spots, 3G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR8085113	30,460,807	3G	1.9Gb	2018-10-23

ID:: 6613430

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