U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX4774141: GSM3405609: Persister cells - cells treated with ciprofloxacin 1; Pseudomonas aeruginosa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 14.6M spots, 2.9G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome Sequence of Antibiotic-Treated Pseudomonas aeruginosa
PRJNA493733 • SRP162817 • All experiments • All runs
show Abstracthide Abstract
Pseudomonas aeruginosa is known to tolerate antibiotic therapy during infection. This prevents clearance of infection and negatively impacts patient outcomes. Here, we report the transcriptome sequence of antibiotic-treated and untreated P. aeruginosa cultures and the differential gene expression observed when treated cells are compared to untreated cells. Overall design: P. aeruginosa antibiotic tolerance, biofilm, planktonic, and stationary cultures
Sample: Persister cells - cells treated with ciprofloxacin 1
SAMN10141784 • SRS3854683 • All experiments • All runs
Organism: Pseudomonas aeruginosa PAO1
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated from untreated and persister cells using the RNeasy Kit (QIAgen) according to manufacturer's instructions and treated twice with TURBO DNase (Ambion, Grand Island, NY) to remove any contaminating DNA (5, 6). Ribosomal RNA was depleted using the Ribo-Zero Magnetic kit (Epicentre, Madison, WI) Directional library was generated using the NEXTflex directional RNA-seq kit (Bioo Scientific, Austin, TX)
Experiment attributes:
GEO Accession: GSM3405609
Links:
Runs: 1 run, 14.6M spots, 2.9G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR793897614,590,0712.9G1.7Gb2018-10-31

ID:
6458346

Supplemental Content

Related information

Search details

See more...

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...