GSM3308708: B73_2_pericarp_1; Zea mays; RNA-Seq - SRA

SRX4476034: GSM3308708: B73_2_pericarp_1; Zea mays; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 53.3M spots, 2.7G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)

Study: The dominant and poor penetrant phenotypes of the maize mutation Unstable factor for orange1 are caused by DNA methylation changes at a linked transposon

PRJNA483200 • SRP155527 • All experiments • All runs

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Transcriptional analysis of the Ufo1-1 mutant allele and wild type plants were used to identify the candidate gene using a k-mer based approach. The transcriptome data also revealed the altered expression of carbohydrate metabolic genes, which translates into elevated levels of soluble sugars in leaves and light-induced lesions found in mature Ufo1-1 leaves. Overall design: Genome-wide RNA and sRNA profiling was done by Illumina TruSeq sample preparation followed by high-throughput sequencing with Illumina HiSeq 2000 platform.

Sample: B73_2_pericarp_1

SAMN09727478 • SRS3601135 • All experiments • All runs

Organism: Zea mays

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Materials were collected and flash frozen in liquid nitrogen. Total RNA was extracted by Tri reagent (Molecular Research Center, Inc.) according to manufacturer's protocol. Small RNA libraries were constructed with Illumina Truseq small RNA sample preparation kit (RS-200-0012). RNAseq libraries were constructed with Illumina Truseq RNA sample preparation kit (RS-122-2001) . Script-Seq libraries (strand-specific RNAseq) were constructed with Illumina ScriptSeq RNA-seq library preparation kit (SSV21106).

Experiment attributes:

GEO Accession: GSM3308708

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 53.3M spots, 2.7G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR7611206	53,325,609	2.7G	1.6Gb	2019-01-23

ID:: 6061925

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