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SRX4400365: GSM3289412: 08_h_dproQ_R2; Homo sapiens; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 29.3M spots, 2.2G bases, 917.2Mb downloads

Submitted by: NCBI (GEO)
Study: Role of ProQ in Salmonella virulence - dual RNA-Seq
show Abstracthide Abstract
FinO-domain proteins such as ProQ of the model pathogen Salmonella enterica have emerged as a new class of major RNA-binding proteins in bacteria. ProQ has been shown to target hundreds of transcripts including mRNAs from many virulence regions but its role, if any, in bacterial pathogenesis has not been studied. Here, using a Dual RNA-seq approach to profile ProQ-dependent gene expression changes as Salmonella infects human cells, we reveal dysregulation of bacterial motility, chemotaxis and virulence genes which is accompanied by altered mitogen-activated protein kinase signaling in the host. Comparison with Hfq, Salmonella's other major RNA chaperone, reinforces the notion that these two global RNA binding proteins work in parallel to ensure full virulence. Of newly discovered infection-associated ProQ-bound small noncoding RNAs, we show that STnc540 represses a virulence-related magnesium transporter mRNA in a ProQ-dependent manner. Together, this comprehensive study uncovers the relevance of ProQ for Salmonella infection and highlights the importance of RNA-binding proteins in regulating bacterial virulence programs. Overall design: Dual RNA-seq or bacterial RNA-seq
Sample: 08_h_dproQ_R2
SAMN09671200 • SRS3554914 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: mirVana kit TruSeq-based (at Vertis Biotech. AG, Freising, Germany)
Experiment attributes:
GEO Accession: GSM3289412
Links:
Runs: 1 run, 29.3M spots, 2.2G bases, 917.2Mb
Run# of Spots# of BasesSizePublished
SRR753241929,304,4902.2G917.2Mb2018-11-28

ID:
5978567

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